- Fungal host-pathogen interactions
- G-protein coupled receptors (GPCRs) in infection and disease.
- Industrial enzyme engineering
On the one hand our lab is currently investigating mechanistic aspects of fungal disease including existing and new potential sources of host-susceptibility and resistance as well as pathogen virulence factors. Fungal pathogens continue to adapt and develop resistance to known sources of host-resistance and fungicides. However with a better understanding of the mechanisms mediating fungal infection we can develop new more innovative and durable sources of resistance and anti-fungal compounds.
In all instances target genes of interest are characterized biochemically in vitro by relevant assays relying on recombinantly produced and enriched gene products. Targets range from cell surface receptors and ABC transporters to related enzymes involved in allocrite/ligand biosynthesis, metabolism as well as signal transduction. Assays use a variety of different analytical methods such as liquid chromatography, mass spectroscopy, photospectroscopy and nuclear magnetic resonance for metabolite analyses. We also use protein crystallography and isothermal titration calorimetry to better understand protein-ligand interactions.
One target of particular interest to us are G-protein coupled receptors that mediate fungal infection. We use cell biology techniques including fungal growth or chemotactic plate assays, combined with mutagenesis, to asses the contributions of GPCR’s to fungal infection and to elucidate mechanisms associated with these roles, and the nature of stimulatory ligands and downstream signalling pathways.
On the other hand we have more recently also become engaged in industrial enzyme engineering toward the development of novel bioprocessing technologies to apply to industrial waste streams for the production of value added products in the natural health products, food colorings and nutrition fields.
Publications, Protein Structures and Patents
Our 3 latest publications include:
Loewen PC, Switala J, Wells JP, Huang F, Zara AT, Allingham JS, Loewen MC (2018) Structure and function of a lignostilbene-α,β-dioxygenase orthologue from Pseudomonas brassicacearum. BMC Biochemistry, 19:8. doi: 10.1186/s12858-018-0098-4. [online]
Demissie ZA, Foote S, Tan Y, Loewen MC. (2018) Profiling of the Transcriptomic Responses of Clonostachys rosea Upon Treatment With Fusarium graminearum Secretome. Frontiers in Microbiology, epub ahead of print. doi.org/10.3389/fmicb.2018.01061. [online]
Cuperlovic-Culf M, Rajagopalan N, Loewen M, Surendra A. (2017) Perspectives on the specific targeting of Fusarium graminearum for the development of alternative head blight treatment approaches. Plant Pathol, 66: 1391–1403. doi:10.1111/ppa.12726. [online]
Current Group Members
Michele C. Loewen
Senior Research Officer
Adjunct Professor – QueensU
Khanh Luu (Ph.D. Student; USASK)
Zerihun Demissie (Research Associate; NRC)
Pooja Sridhar (Ph.D. Student; QueensU)
Stanley Liang (Coop Student; University of Victoria)
Kelly Robinson (Technical Officer; NRC)
Tanya Sharma (Ph.D. Student; uOttawa)
Please see also our lab Alumni